Table 1 Characterization of anti-CD3 Fabs binding to different CD3E/D variants by SPR.
Fab | Human CD3E/D | Cyno CD3E/D | Murine CD3E/D | Chimeric human/ murine CD3E/D |
|---|---|---|---|---|
KD (nM) | KD (nM) | KD (nM) | KD (nM) | |
hCD3.1 | 6.3 ± 0.18 | 6.5 ± 0.11 | NB | 8.6 ± 0.53 |
hCD3.2 | 3.1 ± 0.35 | 3.8 ± 0.61 | NB | 4.2 ± 0.22 |
hCD3.3 | 0.77 ± 0.13 | 0.91 ± 0.17 | NB | 0.81 ± 0.06 |
hCD3.4 | 2.7 ± 0.45 | 2.7 ± 0.02 | NB | 3.1 ± 0.23 |
hCD3.5 | 4.1 ± 0.44 | 5.0 ± 0.5 | NB | 4.4 ± 0.27 |
SP34 | 6.6 ± 0.46 | 7.5 ± 0.06 | NB | 7.3 ± 0.18 |
2C11 | NB | NB | 22.0 ± 1.4 | 250 ± 16 |
