Figure 1
From: Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cells
rVAR2 is internalized into cancer cells. (a) Confocal images of internalized rVAR2 in cancer cell lines after 2 h of treatment. (b) Western blot for rVAR2 in PC3 and COLO205 cells from lysates collected by either direct lysis on the plate or trypsinizing the cells. (c) Confocal images of cells treated with rVAR2 at 4 °C or 37 °C. (d) Analysis of rVAR2 binding with or without ATP depletion in PC3 and COLO205 cells. (e) Representative images from live cell confocal of rVAR2 internalization in COLO205 cells, arrow heads indicate the presence of rVAR2 inside cells. (f) Western blot of rVAR2 accumulation in PC3 and COLO205 cells over time. Vinculin was used as loading control. (g) COLO205 cells were pulse treated with rVAR2-488 and allowed to be internalized for 2 h prior to rVAR2-594. Scale bar represents 5 µm.
