Figure 3
From: Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cells
Endocytic mechanism of rVAR2. (a) Western blot for rVAR2, CHC, and Cav-1 in PC3 and COLO205 cells following transfection with siSCR, siCHC, or siCav-1. Actin was used as loading control. (b) Confocal images of rVAR2 in Triton X-100 treated PC3 and COLO205 cells, transferrin (TFn) was used as control for Triton X-100 treatment. Scale bar represents 10 µM. (c) Colocalization of rVAR-488 and Dextran-594 in COLO205 and PC3 cells at indicated timepoints. Scale bar represents 5 µM. (d) Electron microscopy of COLO205 cells treated with rVAR2. Gold-nanoparticle labeled antibodies was used to detect the presence of rVAR2. Arrowheads indicate the presence of rVAR2 binding near membrane protrusions that corresponds to structures formed during macropinocytosis.
