Figure 5

rVAR2 is released outside cells with exosomes. (a) PC3 and MG63 were treated with rVAR2, trypsinized lysated or media was collected at the indicated timepoints and blotted for the presence of rVAR2. Vinculin was used as loading control for cell samples and ponceau red stain was used as loading control for media samples. (b) Nanoparticle tracking analysis (NTA) of exosomes purified from the media of PC3 or MG63 cells. (c) Proteome of PC3 or MG63 exosomes as determined by mass spectrometry, common proteins present in exosomes from both cell lines are highlighted. CSPGs found in the exosomes of both cell lines are listed and known cell surface CSPGs are listed in blue. (d) Western blot of exosomes from PC3 or MG63 cells 48 h after rVAR2 treatment. 20 billion particles were used for loading and CD9 was used to determine the presence of exosomes.