Figure 5

bcat-1 RNAi-treated worms exhibit cholinergic neurodegeneration. (A,B) Cholinergic neuron projections in the head, ventral nerve cord (VNC), and dorsal nerve cord (DNC) of RNAi-sensitive worms (unc119p::sid-1;unc-17p::GFP) were scored for the degree of degenerated appearance on day 8 following bcat-1 or control RNAi treatment during adulthood. In (A), representative images of DNC (left), VNC (middle), and head neurites (right) are shown, with arrows indicating abnormal neurite regions. Scale bars, 50 μm. Quantification in (B). Chi-square test in, n ≥ 60 worms per group. **p < 0.01; ***p < 0.001, ****p < 0.0001.