Figure 1
From: Absence of DEATH kinesin is fatal for Leishmania mexicana amastigotes
Features of LmxKIN29. (A) amino acid sequence of LmxKIN29 with putative MAP kinase phosphorylation sites underlined (serine 333, threonine 440, serine 548, serine 551, and serine 554), the motor domain (R20-I355) is shown in grey (pfam00225), the DEATH motif (D279–H283) is bold and underlined. R20, R22, P23 (↓), G100-F108 (*, Walker A motif GxxxxGKT/S), the switch-1 motif (NxxSSR)(.) and D261 of the switch-2 motif (DxxGxE)(:) are involved in ATP-binding; R308, K311, and R314 are residues of the microtubule-binding site (#); I355 in bold is involved in neck linker binding; K368 marks the start of the coiled-coil dimerisation domain (^). (B) 3D model of LmxKIN29 using 1goj in SWISS-MODEL and displayed by Jmol FirstGlance 3.0 (http://firstglance.jmol.org) showing the localisation of the neck linker, of the ATP-binding and microtubule-binding sites, and of serine 333. (C) coiled-coil prediction for LmxKIN29 (left) and its T. brucei orthologue (right) using COILS version 2.2 and a window of 21 amino acids. (D) partial amino acid sequence alignment of LmxKIN29 from L. mexicana with the amino acid sequences of various kinetoplastid kinesins. Lmex, LmxM.29.0350 Leishmania mexicana MHOM/GT/2001/U1103; Lama, LAMA_000589400 Leishmania amazonensis MHOM/BR/71973/M2269; Lmaj, LmjF.30.0350 Leishmania major strain Friedlin; Linf, LINF_300008500-T1 Leishmania infantum JPCM5; Ldon, LdBPK_300350.1.1 Leishmania donovani BPK282A1; Laet, LAEL147_000550000 Leishmania aethiopica L147; Ltro, LTRL590_300009000 Leishmania tropica L590; Cfas, CFAC1_260021700 Crithidia fasciculata strain Cf-Cl. The asterisk (*) indicates conserved amino acid residues in all orthologues (alignment by Clustal Omega modified).
