Figure 1

Hematoxylin and eosin (HE) stained specimens. Specimens were stained for HSI imaging standardizedly. Areas with squamous epithelium (A) or EAC cells (C) were selected and recorded by the HSI camera. The corresponding RGB images reconstructed from the HSI data are shown in (B) and (D) (Bg Background, SE Squamous epithelium and EAC Esophageal adenocarcinoma cells).