Figure 9
Flow cytometry of HepG2 cells incubated with 1 μM of IR794 for 15 min in culture media. Cellular trafficking pathway of IR794-Morph and IR794-Morph-Mpip characterised by various inhibitors and at 4 °C condition. The endocytosis inhibitors used include amiloride (micropinocytosis), genistein (caveolin-mediated), Fillipin III, dynasore (clathrin-mediated) and BSP. Data reports as % Medium fluorescence intensity and represents the mean ± SD (n = 3, independent experiment). Statistical analysis is based on Pair Student’s T-test analysis (*P < 0.05, **P < 0.01, ***P < 0.001) where the comparison between the uptake at 37 °C and the uptake in the presence of different inhibitors is marked.
