Figure 1
From: Long-read sequencing for identification of insertion sites in large transposon mutant libraries
Mapping of transposon directed insertion site sequences to repeated nucleotide sequences in a reference genome. Panel (A) depicts the steps from genomic DNA to transposon directed insertion site sequencing and alignment of short-reads, indicating that short-reads cannot be mapped uniquely to repeated sequences. Panel (B) depicts the steps from genomic DNA to transposon directed insertion site sequencing and alignment of long-reads, indicating that long-reads resolve the mapping challenge across repeated sequences.
