Figure 5
From: Long-read sequencing for identification of insertion sites in large transposon mutant libraries
Long-reads mapped uniquely to a single copy of a repeating ribosomal RNA gene cluster of over 5 kb. In the E. coli genome, the longest repeating nucleotide sequences are those of the ribosomal RNA operons, of which there are seven copies, each of over 5 kb in length. These are, therefore, a good test of the ability of LoRTIS to identify insertion sites uniquely within long repeating sequences. A genetic map of the relative rRNA gene positions is shown at the bottom of the panel as blue arrowhead boxes. Above this, each panel depicts the long-reads, each shown as a fine horizontal line, that map uniquely within this repeat element.
