Figure 7
From: Long-read sequencing for identification of insertion sites in large transposon mutant libraries
An overview of LoRTIS sequencing library preparation and alignment. The gDNA was extracted from a high-density transposon insertion library containing multiple insertions in every non-essential genomic locus. The gDNA was tagmented and transposon junctions were amplified using one biotinylated primer followed by another step using two primers for amplification. Biotinylated DNA fragments were selected using streptavidin beads and nested PCR was done to add sequencing primers. The library was sequenced using nanopore and the reads mapped to the reference genome using Bio-LoRTIS software (see “Materials and methods”).
