Table 3 Characteristics of the primers used in quantitative RT-PCR analysis of Cobb 400 and Kadaknath chicken meat.
Role | Gene | Gene name | Primer sequence F, forward (5´ to 3´) R, reverse (3´ to 5´) | Annealing temperature | Product size (bp) | NCBI Gene ID |
|---|---|---|---|---|---|---|
Carnosine synthesis | CARNS1 | Carnosine synthase | F: CTGCCCTGGAAGAATTTGTG | 60 °C | 172 | 100,359,387 |
R: GACAGCAACCAGCGAGAGAG | ||||||
Carnosine hydrolysis | CNDP1 | Carnosinase-1 | F: ATTCTCCATTCGCCAAGTTC | NA | 421,012 | |
R: GCATCTGCATCACCAATAGG | ||||||
CNDP2 | Carnosinase-2 | F: AAACCTTGGGTGTCAGACTT | 149 | 421,013 | ||
R: ACATTCTTGCCTGTTGCTTC | ||||||
β-alanine transport | SLC6A6 | Solute carrier family 6, member 6 | F: GGGAAATCTTCATCGCTAT | 169 | 416,041 | |
R: CCATAAACCCAGGCTAC | ||||||
SLC36A1 | Solute carrier family 36, member 1 | F: CACGGCAGTTCCCTCTGAT | 135 | 770,250 | ||
R: AGCAGTTGGGCAGGTTGAG | ||||||
SLC6A14 | Solute carrier family 6, member 14 | F: AAACACGCCTCGTAAATGA | NA | 100,857,521 | ||
R: CACGATGTTGCCAGTCTCA | ||||||
Reference gene | ACTB | β-actin | F: ATCCGGACCCTCCATTGTC | 120 | 396,526 | |
R: AGCCATGCCAATCTCGTCTT |
