Figure 3

Main effect of 5-Hydroxy-L-tryptophan (5-HTP) dose. Gene expression of primary bovine mammary epithelial cells (pBMEC) cultured with 50, 200 or 500 μM of 5-HTP for 12-h. Data is presented as relative mRNA expression (∆∆Ct = ∆Ct Dose 0 (CON) − ∆Ct Dose 50, 200 or 500)) ± standard error of the mean (SEM). Positive and negative ∆∆Ct indicates upregulation and downregulation, respectively. Significance declared at P value ≤ 0.05 (*) and (#) denotes a statistical tendency at 0.05 < P value ≤ 0.10.