Figure 5 | Scientific Reports

Figure 5

From: In vitro effects of 5-Hydroxy-L-tryptophan supplementation on primary bovine mammary epithelial cell gene expression under thermoneutral or heat shock conditions

Figure 5

Interaction between 5-Hydroxy-L-tryptophan (5-HTP) dose and incubation temperature. Gene expression of primary bovine mammary epithelial cells (pBMEC) after 12-h exposure of either 0, 50, 200 or 500 μM dose of 5-HTP under thermoneutral (37 °C, TN) or heat shock (41.5 °C, HS) culture conditions. Data is presented as relative mRNA expression (∆∆Ct = ∆Ct TN Dose 0 (CON) − ∆Ct treatment)) ± standard error of the mean (SEM). Significance declared at P value ≤ 0.05 (*) and (#) denotes a statistical tendency at 0.05 < P value ≤ 0.10.

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