Figure 7

Analysis of cell migration by in vitro wound healing assay. (a) Time-lapse microscopy images after culture insert removal. Scale bars = 100 μm; (b) Quantification of the wound area after treatment A-549 cells with increasing concertation’s of PCH-1. Data represent mean ± SEM of three independent experiments. Statistical differences were analyzed by one-way ANOVA and post hoc Dunnett’s test. ** p < 0.001, *** p < 0.0001, **** p < 0.00001; (c) Quantification of wound area after treatment TGF-β1 stimulated A-549 cells with increasing concentrations of PCH-1.