Table 2 The list of antibodies used in Western Blot analysis.
From: miR-378 affects metabolic disturbances in the mdx model of Duchenne muscular dystrophy
I° antibody/dilution | Vendor/Cat. No | II° antibody/dilution | Vendor/Cat. No |
|---|---|---|---|
Rabbit anti-mouse pAKT (Ser 473)/1:1000 | Cell Signaling Technology, Danvers, MA, USA/9271S | Goat anti-rabbit/1:10000 | Cell Signaling Technology, Danvers, MA, USA/7074 |
Rabbit anti-mouse AKT/1:1000 | Cell Signaling Technology, Danvers, MA, USA/9272S | Goat anti-rabbit/1:10000 | Cell Signaling Technology, Danvers, MA, USA/7074 |
Mouse anti-porcine GAPDH/1:2000 | Santa Cruz Biotechnology, Dallas TX, USA/sc-59540 | Goat anti-mouse/1:20000 | BD Pharmingen, San Diego, CA, USA/554002 |
Rabbit anti-rat GLUT4/1:1000 | Abcam, Cambridge, UK/ab654 | Goat anti-rabbit/1:10000 | Cell Signaling Technology, Danvers, MA, USA/7074 |
Rabbit anti-human GSK-3β/1:500 | Santa Cruz Biotechnology, Dallas TX, USA/sc-9166 | Goat anti-rabbit/1:5000 | Cell Signaling Technology, Danvers, MA, USA/7074 |
Goat anti-human PPARα/1:500 | Santa Cruz Biotechnology, Dallas TX, USA/sc-1985 | Donkey anti-goat/1:5000 | Thermo Fisher Scientific, Waltham, MA, USA/PA1-28664 |
Rabbit anti-human p110α/1:500 | Cell Signaling Technology, Danvers, MA, USA/4249T | Goat anti-rabbit/1:5000 | Cell Signaling Technology, Danvers, MA, USA/7074 |
Mouse anti-human VINCULIN/1:400 | Sigma-Aldrich, St. Louis, MO, USA/ V9131 | Goat anti-mouse/1:10000 | BD Pharmingen, San Diego, CA, USA/554002 |
