Table 1 Characteristics of 9 SSR loci developed in Chamaecyparis formosensis.
Locus | Primer sequences (5’ -3’) | Repeat motif | Fluorescent label | Allele size (bp) | Ta (°C) | GenBank accession no | Putative function [organism] |
|---|---|---|---|---|---|---|---|
Cred603 | TTGCTACATTAGCACTAGATAGCAAAGAAA | (AAG)13 | 6-FAM | 106 | 60 | MW052386 | No hit |
ACTGAAGATACTGAGGATATTGAAGAGGAA | |||||||
Cred610 | TGAGATATACATGTGTGAAAGAGAGTGAAGC | (GTAT)5 | PET | 166 | 60 | MW052387 | No hit |
TGCAATAATTTCTTCAGTGTTACCACTACC | |||||||
Cred628 | GCTGGAGTCATTATAGTGCCATGTCTTTGT | (GCCC)3 | 6-FAM | 142 | 60 | MW052388 | No hit |
TTTTCAAATAGCCGACCGACCTATGTAGAG | |||||||
Cred640 | ACCCATATCTTCCTTCCCAACCATTAAGAT | (TCTT)5 | 6-FAM | 137 | 60 | MW052389 | No hit |
CTTTCAGTGGAATGGAAGAAAGCCCTACTA | |||||||
Cred641 | ACTTCTAATGAATCCCCATGCCGAATTGTA | (GC)19 | VIC | 193 | 60 | MW052390 | No hit |
CTGTTCGCGATAAGATAATTGGCTAGTGTG | |||||||
Cred674 | TAAAGAGGCTCTGCTACTGGCTTTTCAACT | (GGGC)4 | NED | 147 | 60 | MW052391 | No hit |
GTGGGTGGCCCTCTATTCTATTGTTGAT | |||||||
Cred678 | GGTCCATATCCTGGAGTAGAACCTCCCTAC | (GGGC)5 | PET | 162 | 60 | MW052392 | No hit |
GTGTCGCAGGCATAGACTTCTCCCTATATT | |||||||
Cred682 | CCGCCCTTCTAATAACAGGGAAGATAAGTT | (CCCT)5 | NED | 147 | 60 | MW052393 | No hit |
CCGCCCTTCTAATAACAGGGAAGATAAGTT | |||||||
Cred683 | GCAGCCTAAATAAACAATAGGGGGATTGAT | (GCCT)4 | NED | 146 | 60 | MW052394 | No hit |
CATGTTACGTATAGAATCGAGTGCAGGTCA |
