Figure 1 | Scientific Reports

Figure 1

From: Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment

Figure 1

Screening experimental design. (A) The screening of the oxygenation strategies was done on MPIs encapsulated in alginate macrobeads using a full factorial design 23. The three factors studied and their levels were: w/o or w/HEMOXCell (HEM), w/o or w/silicone-CaO2 (S-O2), and w/ 1% O2 or 20% O2 tension. The response variables assessed after 6 days of culture were the intracellular ATP content per well (RLU), ATP/LDH viability ratio per well (RLU/AU), and the insulin stimulation index. (B) Interaction plots of the screening DoE. Interactions involving HEMOXCell and silicon-CaO2, HEMOXCell and O2 tension, silicone-CaO2 and O2 tension are displayed concerning ATP content, ATP/LDH ratio, and insulin stimulation index.

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