Table 6 Multi-response BAP optimization under hypoxia with and without silicone-CaO2.

From: Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment

Optimization

Optimal level of factors

Response values at the optimum

Factor A HEMOXCell conc

Factor B Islet density

ATP content per device (RLU)

ATP/LDH ratio (RLU/AU)

w/silicone-CaO2

50 µg/mL

3284 IEQ/device

8.73 × 106

≈ 3572 viable IEQa

9.64 × 106

w/o silicone-CaO2

500 µg/mL

375 IEQ/device

2.24 × 106

≈ 656 viable IEQa

3.83 × 106

  1. aNumber of equivalent viable and dead MPIs interpolated from the ATP content standard curve (Supplementary Fig. 3).
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