Figure 5 | Scientific Reports

Figure 5

From: Mitochondrial dysfunction-induced high hCG associated with development of fetal growth restriction and pre-eclampsia with fetal growth restriction

Figure 5

Mitochondrial translation deficiency induced expression of hCGβ and inflammatory cytokine factors. (a) Immunoblot analysis of human chorionic gonadotropin-beta (hCGβ) and growth differentiation factor 15 (GDF15) in JEG3 cells after chloramphenicol (CAP) treatment for 72 h. GAPDH was used as an internal control. The full unedited gels are shown in the Supplementary information (Full unedited gel for Fig. 5). (b,c) Western blot quantification of hCGβ and GDF15. (d) hCGβ mRNA expression in JEG3 cells after CAP treatment for 72 h. (e–h) TNFα and IL8 mRNA expression in JEG3 cells after CAP (e,f) and doxycycline (DOXY; g,h) treatment for 72 h. (b–d) Values are presented as the median with an interquartile range of three independent experiments. Statistical significance was assessed by the Kruskal–Wallis test. *p < 0.05, **p < 0.01. (e–h) Values are presented as the median with an interquartile range of four or five independent experiments. Statistical significance was assessed by Mann–Whitney test. *p < 0.05, **p < 0.01.

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