Figure 2
From: Activation of lysosomal mediated cell death in the course of autophagy by mTORC1 inhibitor
SDS-203 initiates autophagy in l MIA PaCa-2 cells. (A, B) Representative fluorescent images of transiently transfected (GFP-LC3) pancreatic cancer cells treated with SDS-203 (20 µM) for 6, 12, 24 h and rapamycin (200 nM) as a positive control. Quantification of 30 random cells were taken for each experiment. (C–F) Represents protein expression levels of LC3-II in MIA PaCa-2 cells when treated with various concentrations (0, 5, 10, 20, 30 μM; 24 h) or different time points (0, 6, 12, 24 h; 20 µM) of SDS-203. (G, H) MIA PaCa-2 cells were subjected to SDS-203 (20 μM) in a time-dependent manner and expression of p62, ATG5, ATG7, Beclin-1 were analyzed by western blotting. Densitometry quantification of protein expression was done by using ImageJ software. All data are presented as the mean of SD from 3 independent experiments. Statistics, Student’s t test *p < 0.05, **p < 0.01, ***p<0.001 vs. control (0.1%DMSO).
