Figure 1 | Scientific Reports

Figure 1

From: Piezo2 expression and its alteration by mechanical forces in mouse mesangial cells and renin-producing cells

Figure 1

Gene expression and localization of Piezo2 in the kidneys of adult C57BL/6J mice. (a) RNAscope in situ hybridization of Piezo2. Signals were visualized as brown dots by DAB chromogen detection. Piezo2 is expressed specifically in the glomerular and juxtaglomerular regions (arrows). The right panel shows a magnified view of the boxed area in the left panel. Arrowheads indicate vessels. cor, cortex; med, medulla. Scale bars, 100 µm. (b–e) Fluorescence double RNAscope in situ hybridization of Piezo2 (red) and cell markers (green), Pdgfrb (a mesangial cell marker), Nphs1 (a podocyte marker), Tek (an endothelial cell marker), and Ren1 (a marker for renin-producing cells). Arrows indicate Piezo2-expressing Pdgfrb-positive mesangial cells. Arrowhead indicates Piezo2-expressing Ren1-positive renin-producing cells. Scale bars, 100 µm. (f) Quantitative RT-PCR analysis of Piezo2 in the kidney, cortex, medulla, and glomerular (Glom) fractions. Piezo2 expression in the kidney is arbitrarily expressed as 1. Bars represent mean ± s.e.m. **P < 0.01 vs. kidney, one-way ANOVA followed by Bonferroni's post hoc test (n = 4 per group).

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