Figure 2

Expression and localization of Piezo2 protein in the kidneys of Piezo2-GFP-IRES-Cre (Piezo2GFP) reporter mice. (a) Fluorescence immunostaining of GFP (green) representing Piezo2 protein expression in the kidneys of Piezo2GFP reporter (left) and wild-type (right) mice. Scale bar, 100 µm. (b–e) Fluorescence double immunostaining of GFP (green) and cell markers (red), Pdgfrb, Nphs2 (a podocyte marker), Pecam1 (an endothelial cell marker), and Ren1. Scale bars, 100 µm. (f) GFP immunostaining (green) and RNAscope in situ hybridization of Piezo2 (red) in the adjacent serial sections. Scale bar, 100 µm.