Figure 4 | Scientific Reports

Figure 4

From: Cellobiose phosphorylase from Caldicellulosiruptor bescii catalyzes reversible phosphorolysis via different kinetic mechanisms

Figure 4

Kinetic mechanisms of the phosphorolytic reaction. (A) Double reciprocal plots of cellobiose phosphorolysis catalyzed by CbCBP. The concentration of Pi was as follows: 1 mM (filled square), 2 mM (open square), 4 mM (filled Circle), 8 mM (open circle), and 12 mM (Filled triangle). R2 > 0.99. (B–E) Inhibition patterns of the products against the substrates. The other substrates were 1 mM Pi (B, D) and 2 mM cellobiose (C, E). B: Glu-1P against cellobiose, C: Glu-1P against Pi, D: glucose against cellobiose, E: glucose against Pi. filled square and open square: none of the initial product, filled Circle: 2 mM Glu-1P, fillied triangle: 4 mM Glu-1P, open circle: 1 mM glucose, open triangle: 3 mM glucose.

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