Figure 5 | Scientific Reports

Figure 5

From: Cellobiose phosphorylase from Caldicellulosiruptor bescii catalyzes reversible phosphorolysis via different kinetic mechanisms

Figure 5

Kinetic mechanisms of the synthetic reaction. (A) Double reciprocal plots of the synthetic reaction catalyzed by CbCBP. The concentrations of Glu-1P were as follows: 2 mM (filled square), 3 mM (open square), 4 mM (filled circle), 5 mM (open circle), 8 mM (Filled triangle) and 12 mM (open triangle). 0.98 < R2 < 0.99. (B) v-[D-glucose] Plot of the synthetic reaction of CbCBP at fixed concentrations of Glu-1P. Glu-1P was used at 1 mM (filled square), 2.5 mM (open square), 5 mM (filled circle) and 10 mM (open circle) concentrations. The theoretical lines were obtained by regressing the experimental data to the Michaelis Menten equation.

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