Table 2 Kinetic parameters for the reaction of CbCBP with various substrate.

From: Cellobiose phosphorylase from Caldicellulosiruptor bescii catalyzes reversible phosphorolysis via different kinetic mechanisms

Reaction

KmA (mM)

KmB (mM)

Kcat (s-1)

KiA (mM)

Phosphorolysisa

2.8

1.2

18.4

3.3

Syntheseb (A)

35

116.3

294

noc

  1. aA represents cellobiose, and B represents inorganic phosphorus.
  2. bA represents D-glucose 1-phosphate, and B represents D-glucose.
  3. cKiA value could not be calculated because there was no inhibition when the concentration of glucose reached 100 mM in the synthetic reaction.
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