Table 1 Robustness of the analysis methods.

From: Analysis tools for single-monomer measurements of self-assembly processes

 

Dwell-time analysis

Visitation analysis

Average-rate analysis

Recommended conditions for distingushing

The mechanism

Single steps

The mechanism

Single steps

The mechanism

Single steps

SNR

> 1

> 1

> 0.1

> 1

> 0.1

> 0.1

Sampling rate

> 10×a

> 20×

> 1×

> 2×

> 1×

> 5×

LE (stochastic)

> 0.7

> 1

< 1

1

> 0.3

> 0.7 (1b)

LE (specific)

> 0.3

1

> 0.3

1

> 0.3

1

Photobleaching

< 3%a

< 0.1%

3%

< 2%

< 3%

< 2%

  1. aCompared to the fastest rate.
  2. b1 % to obtain the correct conversion site.