Figure 1

miRNA biogenesis gene expression is elevated in developing TA muscles. (a,b) qPCR analysis of pri-miRs 133b and 206 (a) and Ago2 mRNA (b) expression. (c,d) Western blot analysis of Ago2 protein levels. Images are cropped from the full-length blot, available in Fig. S5. (e) qPCR analysis of DGCR8, Exportin-5 and Dicer mRNA expression. *p < 0.05 versus P1. †p < 0.05 versus P6. One-way ANOVA with Bonferroni post-hoc. All values reported as mean ± SD; n = 3.