Figure 3
Ago2 expression and distribution is altered in the TA following fibular nerve crush injury. (a) qPCR analysis of Ago2 mRNA expression. (b,c) Western blot analysis of Ago2 protein levels. Images are cropped from the full-length blot, available in Fig. S6. (d,e) Representative images of Ago2 IHC in TA cross sections collected from uninjured leg (e) and at 7 days post-injury. (f) Quantification of synaptic Ago2 pixel intensity at 4, 7, and 9 days post-injury, relative to non-synaptic Ago2 pixel intensity. *p < 0.05 versus uninjured leg, One-way ANOVA with Bonferroni post-hoc (a,b) or unpaired 2-sided t test (d). All values reported as mean ± SD; n = 3. Scale bar = 10 µm.
