Figure 4
Ago2 expression and distribution are disrupted in SOD1G93A muscle. (a) qPCR and (b) Western blot analysis of Ago2 levels in TA muscle collected from P70, P90, and P110 SOD1G93A and control littermates (N = 4). mRNA levels are relative to age-matched controls. Protein levels are normalized to total protein (Fig. S7c,f,i). Images are cropped from the full-length blot, available in Fig. S7. (c) Mean synaptic Ago2 pixel intensity in TA muscle fibers of P90 SOD1G93A and control littermates, reported as the ratio of synaptic versus non-synaptic pixel intensity within a muscle fiber (N = 3). (d,e) Representative images of Ago2 (green) distribution at fBTX-labeled NMJs (red) in TA cross sections of P90 control (d) and SOD1G93A (e) littermates. *p < 0.05, versus age-matched control, unpaired 2-sided T-test. All values reported as mean ± SD; n = 4 for all panels except C, where n = 3. Scale bar = 10 µm.
