Figure 1
modified from the open source Servier Medical Art (smart.servier.com) (B) Bar graphs summarizing the number of proteins (top) and phosphorylated peptides (bottom) measured for each heart sample. (C) Principal component analysis of measured protein intensities for all analyzed heart samples. Data from the four animal groups are illustrated by colors: data from animals in the SHAMVh group are depicted in blue, SHAMTr in green, TACVh in red and TACTr in yellow.
Experimental design and proteomics workflow. (A) Mice received either a loose ligature around the aorta (SHAM) or a tight ligature (TAC). Eight weeks post-surgery, cardiac function was evaluated by echocardiography and osmotic pumps delivering either saline (SHAMVh or TACVh) or ß-AR blocker and ACE inhibitor (SHAMTr and TACTr) were implanted. Ten weeks post-surgery, cardiac function was again evaluated by echocardiography, mice were sacrificed, hearts explanted, and the left ventricle dissected. Proteins were extracted from left ventricular tissues and subjected to deep proteome and phosphoproteome measurements by LC–MS/MS analysis. Heart and animal illustrations have been downloaded and
