Figure 6
Chronogram showing divergence estimates (in brackets; Mya, 95% high posterior density limits (HPD) as confidence intervals), based on a relaxed, lognormal clock, Yule diversification model, 5 gene partitions (COI + CYB + H3, 1st codon/COI + CYB, 2nd codon/16S/28S/18S + H3, 2nd codon) and 5 independent clock models for the anchialine Atyidae clade. Stars at nodes indicate calibration points used in BEAST2 analyses. Stochastic mapping of salinity trait evolution is visualized with the probability density of each state at internal nodes. Equal rates and unequal estimation (i.e., 0.75 likelihood of a low salinity ancestor) was assumed at the root. Circles at tips denote salinity preferences of lineages, low salinity (black; state 1) and high salinity (red; state 2). Uncertainty of ancestral states indicated at nodes.
