Figure 1
Illustrations of the two used study designs. (A) In study design 1, C57bl/6 mice were randomized to cages containing running wheels for 4 weeks prior to tumor inoculation and during tumor challenge (EX) or no running wheel (CON), and further randomized to chow or high-fat feeding (HF) (n = 12). (B) In a parallel study (study design 2), mice were randomized to cages containing running wheels for 4 weeks prior to tumor inoculation and during tumor challenge (4 + 2), 4 weeks just prior to tumor inoculation (4 + 0), 2 weeks during tumor challenge (0 + 2), or no running wheel (0 + 0) (n = 12), while either receiving a HF or standard chow diet.
