Figure 2
Effect of wheel running on B16 melanoma growth in mice fed chow or high-fat diet. C57bl/6 mice were randomized to cages containing running wheels for 4 weeks prior to tumor inoculation and during tumor challenge (EX) or no running wheel (CON), and further randomized to chow or high-fat feeding (HF) (n = 12, study design 1). In these mice, (A) tumor volume, (B) body weight, (C) running distance, and (D) weight of the heart were determined at termination, while (E) the average daily food intake was monitored both prior and after tumor induction. In a parallel study (study design 2), mice were randomized to cages containing running wheels for 4 weeks prior to tumor inoculation and during tumor challenge (4 + 2), 4 weeks just prior to tumor inoculation (4 + 0), 2 weeks during tumor challenge (0 + 2), or no running wheel (0 + 0) (n = 12). In these mice, (F) tumor volume, (G) change in body weight, (H) association between tumor volume and change in body weight, (I) the average daily food intake (including the period both prior and after tumor induction) and (J) percentage of necrotic area in B16 tumors from (2F) were determined. Statistical significance was determined by 2-way ANOVA with Tukey’s post hoc test (A, B, D, E, F, G, I, J) or linear regression analysis (H). P values for diet and EX describe the results of the 2-way ANOVA, while $ indicates statistical significance difference between Chow (0 + 0) and HF (0 + 0) at p < 0.05 and individual stars indicate difference from control (CON/0 + 0) group within each feeding group in the post hoc tests. **p < 0.01, ***p < 0.001.
