Figure 2 | Scientific Reports

Figure 2

From: Loss of KAP3 decreases intercellular adhesion and impairs intracellular transport of laminin in signet ring cell carcinoma of the stomach

Figure 2

Morphological changes and decreased cell aggregation in KAP3 KO cells. (A) Immunoblot analysis of KAP3 in KAP3 wild-type (WT) and knockout (KO) cells derived from MNK74 cells. β-actin was blotted as an internal (loading) control. (B) Phase contrast images of KAP3 WT and KO cells. The insets are magnified images of the respective micrographs. Original magnification, × 40. (C,D) Cell aggregation assay. Phase contrast images (C) and the percentage (D) of cell aggregation of KAP3 WT and KO cells after 10 days of culturing in non-adherent dishes. Values are presented as mean ± SD. *P < 0.05. Original magnification, × 40; scale bars, 500 µm. (E) Cell viability assay of KAP3 WT and KO cells cultured in non-adherent dishes (n = 5). Values are presented as mean ± SD.

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