Table 4 Primer sequences utilized for rRT-PCR analysis of targeted gene expression.
Target gene | Primer sequence (5′–3′) | Accession No./Reference |
|---|---|---|
iNOS | F-ACCTTCCGGGCAGCCTGTGA R-CAAGGAGGGTGGTGCGGCTG-3′ | NM_012611 |
COX-2 | F-GCTCAGCC ATACAGCAAATCC R-GGGAGTCGGGCAAT CATCAG | NM_017232 |
Caspase-3 | F-GCAGCTAACCTCAGAGAGACATTC R-ACGAGTAAGGTCATTTTTATTCCTGACTT | NM_012922 |
Bcl-2 | F-TGCGCTCAGCCCTGTG R-GGTAGCGACGAGAGAAGTCATC | NM_016993 |
BAX | F-CAAGAAGCTGAGCGAGTGTCT R-CAATCATCCTCTGCAGCTCCATATT | NM_017059 |
Cytochrome C | F-TTTGAATTCCTCATTAGTAGCTTTTTTGG R-CCATCCCTACGCATCCTTTAC | NM_012839 |
IL-1β | F-TGACAGACCCCAAAAGATTAAGG R-CTCATCTGGACAGCCCAAGTC | NM_031512.2 |
IL-6 | F-CCACCAGGAACGAAAGTCAAC R-TTGCGGAGAGAAACTTCATAGCT | NM_012589.2 |
IL-10 | F-GCCCAGAAATCAAGGAGCATT R-CAGCTGTATCCAGAGGGTCTTCA | L02926.1 |
TNFα | F-CAGCCGATTTGCCATTTCA R-AGGGCTCTTGATGGCAGAGA | L19123.1 |
TGF-β | F-CCAGCCGCGGGACTCT R-TTCCGTTTCACCAGCTCCAT | NM_021578.2 |
β-actin | F-CGCAGTTGGTTGGAGCAAA R-ACAATCAAAGTCCTCAGCCACAT | V01217.1 |
GAPDH | F-TGCTGGTGCTGAGTATGTCG-3′ R-TTGAGAGCAATGCCAGCC-3′ | NM_017008 |
Bacteroides spp. | F:GAG AGG AAG GTC CCC CAC R:CGC TAC TTG GCT GGT TCA G | Layton et al.94 |
Firmicutes spp. Guo et al. 2008 | F-GGAGYATGTGGTTTAATTCGAAGCA R-AGCTGACGACAACCATGCAC | Guo et al.95 |
Enterobacteriaceae | F-CATTGACGTTACCCGCAGAAGAAGC R-CTCTACGAGACTCAAGCTTGC | Bartosch et al.96 |
Bifidobacterium spp. | F-GCG TCC GCT GTG GGC R-CTT CTC CGG CATGGT GTTG | Requena et al.97 |
Bacillus spp. | F-GCA ACG AGC GCA ACC CTT GA R-TCA TCC CCA CCT TCC TCC GGT | Zhang et al.37 |
