Figure 1
From: Alginate oligosaccharides enhance diffusion and activity of colistin in a mucin-rich environment
Optimization of colistin detection and quantification using high-performance liquid chromatography-fluorescence detection. (a) A typical chromatogram showing colistin detection in a polymyxin B spiked sample following colistin diffusion through artificial sputum (AS) medium. (b) Calibration curve of colistin. (c) Colistin quantification in the lower Transwell compartment after diffusion through AS medium ± OligoG at 0.5, 1 and 2% added to the surface of AS medium or pre-incubated for 4 h (± SD; n = 3). Abbreviations: PBS (black), AS (blue), 1% OligoG (surface treatment; orange), 0.5% OligoG (4 h pre-incubation; pink), 1% OligoG (4 h pre-incubation; green), 2% OligoG (4 h pre-incubation; burgundy).
