Table 1 Percentage of the original colistin dose detected in the lower Transwell compartment after 48 h diffusion through artificial sputum (AS) medium ± OligoG using a high-performance liquid chromatography-fluorescence detection method. Significant difference is indicated by *, where *p < 0.05 compared to OligoG untreated control.
From: Alginate oligosaccharides enhance diffusion and activity of colistin in a mucin-rich environment
Artificial sputum treatment | % of colistin original dose | ||||
|---|---|---|---|---|---|
2 h | 4 h | 6 h | 24 h | 48 h | |
PBS (no AS medium) | 59.0 | 65.5 | 68.6 | 88.9 | 94.5 |
No OligoG | 7.9 | 8.2 | 9.2 | 12.5 | 5.6 |
1% OligoG (surface treatment) | 9.3 | 8.2 | 7.1 | 14.4 | 7.1 |
0.5% OligoG (4 h pre-incubation) | 6.6 | 6.3 | 6.3* | 14.9* | 13.6* |
1% OligoG (4 h pre-incubation) | 6.3 | 5.7 | 5.9* | 13.8 | 15.1* |
2% OligoG (4 h pre-incubation) | 8.0 | 8.6 | 11.0 | 19.8* | 20.8* |
