Figure 6

Differentially expressed genes and GO terms of differentially expressed genes between anti-CD80/86 injection and control corneal grafts. (a) Volcano plot of differentially expressed genes (DEGs) of corneal grafts between the anti-CD80/86 and phosphate-buffered saline (PBS) injection groups (control). The gene sets with a false discovery rate (FDR) of less than 0.01 were used as a threshold to determine the significance of DEGs. Green dots represent DEGs, and red dots indicate transcripts that did not significantly change between the anti-CD80/86 and PBS injection groups (control). (b) Hierarchical clustering of DEGs between the anti-CD80/86 injection group and the PBS injection group (control). The X axis represents the two compared samples (anti-CD80/86 injection and PBS injection group). The Y axis represents DEGs. The color (from blue to red) represents gene expression intensity from low to high. (c) The bubble plot of Gene Ontology terms. The z-score is assigned to the X axis and the negative logarithm of the P value to the Y axis, as observed in the bar plot (the higher the more significant). The area of the displayed circles is proportional to the number of genes assigned to the terms. Only the immunologically enriched GO terms’ labels are displayed.