Figure 3

Anti-human CD137 antibody-binding cells in LLC1/OVA/hGPC3-bearing hCD137-KI mice. An Alexa Fluor 488-labeled isotype control antibody or Ure-MB was administered twice at a dose of 1 mg/kg. The tissues were sampled the day after the second administration. (A) Representative data plot of Alexa Fluor 488-labeled antibody-binding CD45⁺ cells in tissues. The population in the square represents Alexa Fluor 488-positive cells. (B) Percentage of Alexa Fluor 488-labeled antibody-binding CD45⁺ cells. Alexa Fluor 488-positive cells were detected in each tissue. The data were collected from three mice in each group. *p < 0.05 by t test. (C,D): (C) Alexa Fluor 488-labeled isotype control antibody binding and (D) Alexa Fluor 488-labeled Ure-MB binding for each type of cell in the tumor and spleen. The percentage of Alexa Fluor 488-positive cells for each cell type was calculated by flow cytometry. #NK cells in tumors were not detected in this experiment.