Figure 3

(A, B, C): Pathway analysis of different metabolites. The results of metabolic pathway analysis are shown as a rectangular tree. Each block in the rectangular tree represents a metabolic pathway, and the block size represents the influential factors of the pathway in the topological analysis. The larger the block size, the larger the influential factor; the block color represents the p value of enrichment analysis (taking a negative natural logarithm, i.e. -ln (P)). The darker the color, the smaller the P value, and the more significant the degree of enrichment. N: normal control group, D: varicocele group, T: varicocelectomy group. (D, E, F): Regulatory network analysis of differentially expressed metabolites. The red dot represents a metabolic pathway, the yellow dot represents a substance related to a regulatory enzyme, the green dot represents the background material of a metabolic pathway, the purple dot represents the molecular module (class), the blue dot represents a chemical interaction reaction, and the green dot represents the differentially expressed substances. N: normal control group, D: varicocele group, T: varicocelectomy group. The treemap plot is generated by R(KEGGgrph,treemap) software and the version is 1.46.0, 2.4–2. The network plot is generated by R(network,igraph) software and the version is 1.16.1,1.2.6.