Figure 4 | Scientific Reports

Figure 4

From: Molecular characterization of strawberry vein banding virus from China and the development of loop‑mediated isothermal amplification assays for their detection

Figure 4

Optimization of the LAMP system to detect SVBV. (a) temperature: 60, 61, 62, 63, 64, 65 °C; (b) time: 30, 45, 60, 75 min; (c) primers FIP/BIP: 1.0, 1.2, 1.4, 1.6, 1.8 μM; (d) primers F3/B3: 0.1, 0.15, 0.2, 0.25, 0.3 μM; (e) Mg2+: 2.0, 4.0, 6.0, 8.0, 10.0 mM; (f) dNTPs: 0, 0.4, 0.8, 1.2, 1.6, 2.0 mM; (g) Betaine: 0, 0.6, 0.8, 1.0, 1.2, 1.4 M; lane M, DL 2000 DNA marker (100–2000 bp). The grouping of gels cropped from different gels were divided with white space. The full-length gels are presented in Supplementary Fig. S4.

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