Figure 6

BT1 binds to hyperphosphorylated and oligomeric tau in OA treated neurons. (A) Bar charts showing the fluorescence intensity quantification of (left) T22 signal (**p = 0.002, MW test; n = 53/3, fields of view/batches) and (right) AT8 signals (****p < 0.0001, MW test; n = 53/6/3, fields of view/batches) in control condition and after the treatment with okadaic acid (50 nM) for 2 h. (B) Left, Manders’s colocalization in-dex of T22 staining with TAU1 (green) and BT1 (orange) fluo-rescence signal in control condition (p = 0.48, t-test; n = 25/3, fields of view/batches) and after the treatment with okadaic acid (50 nM) for 2 h (*p < 0.017, MW test; n = 25/3, fileds of view/batches). Right, Manders’s colocalization index of AT8 staining with TAU1 (green) and BT1 (orange) fluorescence signal in control condition (p = 0.408, MW test; n = 25/3, fields of view/batches) and after the treatment with okadaic acid (50 nM) for 2 h (****p < 0.0001, MW test; n = 25/3, fileds of view/batches), as determined using the custom-made MATLAB code. (C) On the left, Pearson’s correlation index of T22 staining with TAU1 (green) and BT1 (orange) fluorescence signal in control condition (***p = 0.0008, t-test; n = 25/3, fields of view/batches) and after the treatment with okadaic acid (50 nM) for 2 h (p = 0.423, t-test; n = 25/3, fileds of view/batches). On the right, Pearson’s correlation index of AT8 staining with TAU1 (green) and BT1 (orange) fluorescence signal in control condition (p = 0.056, MW test; n = 25/3, fields of view/batches) and after the treatment with okadaic acid (50 nM) for 2 h (****p < 0.0001, MW test; n = 25/3, fileds of view/batches), as determined using the custom-made MATLAB code. (Matlab software, version 2021a; URL: https://it.mathworks.com/products/matlab.html?s_tid=hp_products_matlab).