Figure 6
SnAN2 transcription factor activates flavonoid biosynthetic genes. (a) Comparison of SnAN2 and SlAN2 expression. L, leaf; SA, shoot apex; MF, mature fruit. Data are shown as mean ± standard deviation. N.D, not detected. (b) Representative image of wild-type (WT) and two independent snan2-cr mutants for T1 generation. Scale bar, 1 cm. (c) Gene structure of WT and alleles of the snan2-cr mutants. Red and three bold characters, each target (sgRNA) and protospacer adjacent motif (PAM) site. Blue characters, each mutation. (d) qRT-PCR results of downstream genes regulated by SnAN2. Expression levels of each gene were normalized with the value of WT and UBIQUITIN was used as the endogenous control. Data are shown as mean ± standard deviation: n = 4, biological replicates. N.D, not detected. (e) Proposed model for the molecular regulation of flavonoid biosynthesis in the fruits of S. nigrum. Red arrows (left) indicate the positive regulation shown in this study. Red dashed-arrow and blue dashed-line (right) represent predicted positive and negative regulations. TF, transcription factor. C8/9, Cluster 8 and 9 defined in Fig. 1.
