Table 1 Details of primers used and designed in the current study.
Primer name | Sequence | Annealing temp. (°C) | Product length | Reference or source |
|---|---|---|---|---|
16Sar-F | CGC CTG TTT AAC AAA AAC AT | 60 | 550 +  | Palumbi87 |
16Sar-R | CCG GTC TGA ACT CAG ATC ACG T | |||
Allopaa1-F | TCT GCC TGT TGG TTT TGG GT | 60 | 839 | This work |
Allopaa1-R | CAC GTC CTC AGG AAC CAG TC | |||
Allopaa2-F | TAT CAA CGG CAT CAC GAG GG | 60 | 929 | This work |
Allopaa2-R | CTG TTC ATC CCT GCG TTC CA | |||
Allopaa3-F | CCG TGA AGA AGC GGG GAT AA | 60 | 880 | This work |
Allopaa3-R | TGC ATC GCT CCC TGT TCA TC | |||
Allopaa4-F | TAG CAC GAA AAT TCT GCC TGT | 60 |  ~ 50 | This work |
Allopaa4-R | TCC GTT CAT CGT GGA GGT TTA | |||
Allopaa5-F | CCA CAA AAT TCT GCA TAG CAC G | 60 | 96 | This work |
Allopaa5-R | GTT AAT TCC GTT CAT CGT GGA G | |||
Nanorana 1-F | CGT GAA GAA GCG GGG ATG AA | 60 | 437 | This work |
Nanorana 1-R | CGA CTC GTC AGC TGA GAA CA | |||
Nanorana 2-F | ACA GTG AGA CAA GCT ACG CA | 60 | 103 | This work |
Nanorana 2-R | TGC AGC ATC TAC GTC AGC AC | |||
Nanorana 3-F | AGC TGA CGA GTC GAG TCA AA | 60 | 211 | This work |
Nanorana 3-R | GCG ATC TGC AGA CAA CTA CG | |||
Nanorana -4 F | CAG CTG ACG AGT CGA GTC AA | 60 | 149 | This work |
Nanorana -4 R | ATG CAG CAT CTA CGT CAG CA | |||
Nanorana -5 F | AGA CCC CAT GGA GCT TCA AA | 60 | 454 | This work |
Nanorana -5 R | TGC GTA GCT TGT CTC ACT GT | |||
Nanorana 6-F | GAC CCC AGG CTA TGC AGA AT | 60 | 114 | This work |
Nanorana 6-R | GGA AAA CTA TTT CCC CCG GA | |||
Nanorana 7-F | AAA CAG GAC CCC AGG CTA TG | 60 | 123 | This work |
Nanorana 7-R | AAA GGA AAA CTA TTT CCC CCG GA | |||
Nanorana 8-F | ATT CTT AGG GTG GTG GCC CT | 60 | 118 | This work |
Nanorana 8-R | ATT CTG CAT AGC CTG GGG TC | |||
Nanorana 9-F | GGG TGG TGG CCC TCA GAT TA | 60 | 103 | This work |
Nanorana 9-R | TAG CCT GGG GTC CTG TTT GT | |||
Nanorana 10-F | GAC CCC ATG GAG CTT CAA AC | 60 | 65 | This work |
Nanorana 10-R | GGG CCC CCG GTT TTT CAA | |||
Nanorana 11-F | CGT GAA GAA GCG GGG ATG AA | 60 | 98 | This work |
Nanorana 11-R | GCC CCC GGG TAA GGA AAA T | |||
Nanorana 12-F | TGA AGA AGC GGG GAT GAA TCT | 60 | 95 | This work |
Nanorana 12-R | CCC CCG GGT TAG CCA AA | |||
Nanorana 13-F | TGA AGA AGC GGG GAT GAA TCT A | 60 | 96 | This work |
Nanorana 13-R | GCC CCC GGG TTA GCC A | |||
Nanorana 14-F | GTG AAG AAG CGG GGA TGA AT | 60 | 99 | This work |
Nanorana 14-R | GGG CCC CCG GGT TAG |
