Figure 3 | Scientific Reports

Figure 3

From: Mutations of the DNA repair gene PNKP in a patient with microcephaly, seizures, and developmental delay (MCSZ) presenting with a high-grade brain tumor

Figure 3

Cellular localization of PNKP. (A) HeLa PNKP−/− cells were stably transfected with indicated GFP-tagged PNKP constructs (green), tubulin immunofluorescence (red) indicates the cytoplasmic area, Hoechst staining (blue) indicates the nuclear area. Images were captured using an LSM710 confocal microscope. (B,C) PNKP protein expression is restricted to the nucleus in normal tissue (b—Testes, c—Cerebellum. I—Molecular layer, ii—Purkinje layer, iii—granular layer). (D,E) PNKP expression in the patient derived tumor tissue is nuclear and cytoplasmic (higher magnification image with back arrows in E).

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