Figure 4

Inhibition of exportin 1 leads to nuclear retention of P101L PNKP. Stably-transfected PNKP-knockout HeLa cells expressing GFP-tagged wild-type and mutant PNKP were treated with leptomycin B (LMB) for 3 h and then stained with DAPI and imaged at 40 × magnification.