Figure 5 | Scientific Reports

Figure 5

From: Mutations of the DNA repair gene PNKP in a patient with microcephaly, seizures, and developmental delay (MCSZ) presenting with a high-grade brain tumor

Figure 5

Influence of PNKP variants on cellular response to radiation and DNA repair. (A) Viability of wild-type and mutant cell lines at different radiation dosages. Plots represent mean \(\pm \hspace{0.17em}\)SD. Ordinary one-way ANOVA was performed to compare each cell line with the wild-type HeLa at the same radiation dose using GraphPad Prism 7.0. (B) Single-strand break repair measured by the single cell gel electrophoresis assay. DNA tail moments of cell lines were measured at 1 and 24 h after irradiation. Each column includes data from at least 300 cells. Plots represent mean ± SEM. At each time point, one-way ANOVA was performed to compare each group with the wild-type un-transfected HeLa cells at the same time point using GraphPad Prism 7.0. (C) Repair of DNA double strand breaks by the NHEJ pathway based on 53BP1 foci numbers observed in the wild-type and stably transfected cell lines at different times after irradiation, n indicates the measured cell numbers in each group. The plots represent mean ± SD. In each graph, one-way ANOVA was performed to compare 53BP1 foci at each time point with the number of foci in unirradiated cells using GraphPad Prism 7.0, GraphPad Software. ***p < 0.001, ****p < 0.0001.

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