Figure 6 | Scientific Reports

Figure 6

From: Novel missense ACAN gene variants linked to familial osteochondritis dissecans cluster in the C-terminal globular domain of aggrecan

Figure 6The alt text for this image may have been generated using AI.

Decreased levels of fOCD variant aggrecan protein in patient cartilage. Cartilage tissue explants from a patient heterozygous for the fOCD-linked p.V2455M ACAN variant were obtained at arthroplasty. Some explants were treated with interleukin-1 and oncostatin M to stimulate protein production. After explant culture, the tissue explants were homogenized and extracted, and conditioned medium was collected. After GluC digestion, wild type and p.V2455M variant-specific peptides were quantified by mass spectrometry (MRM LC-MSMS) in the explant extracts (a) and medium (b). Note the decreased levels of p.V2455M variant compared to wild-type aggrecan proteoglycan in explant tissue (a) as well as in the culture medium (b).

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