Figure 1
From: Tissue clearing may alter emission and absorption properties of common fluorophores
BABB alters the fluorescent emission spectra of fluorophores and fluorescent dyes. Plate reader-based emission spectra of Alexa Fluorophores and DAPI in PBS (a) vary from those in BABB (b). The line graphs (c–j) represent the individual emission signals shown in (a,b): Raw emission data visualize the differences in the amplitude between measurements in PBS (black line) and BABB (grey line, c–f). Emission data normalized to their peak intensity highlight the shift of the emission peaks to longer wavelengths (g–j). DAPI was excited at 405 nm (c), Alexa Fluor 488 at 488 nm (d), Alexa Fluor 568 at 555 nm (e), and Alexa Fluor 647 at 639 nm (f). In order to visually distinguish individual spectra, the gaps resulting from missing data points (see “Methods” section) were closed by vertical lines. Error bars indicate standard deviation. n = 8 independent wells for each datapoint.
